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neb 10 beta electrocompetent cells  (New England Biolabs)


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    New England Biolabs neb 10 beta electrocompetent cells
    Neb 10 Beta Electrocompetent Cells, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 652 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/10+beta+electrocompetent+e+coli+cells/NEB+10-beta+Electrocompetent+E%2Ecoli/pm42804500-295-20-20
    Average 99 stars, based on 652 article reviews
    neb 10 beta electrocompetent cells - by Bioz Stars, 2026-10
    99/100 stars

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    Mutagenesis:

    Article Title: The genetic architecture of an allosteric hormone receptor.
    Article Snippet: .. All four PYL1 mutant libraries were electrically transformed into high-efficiency, 10-beta electrocompetent E. coli cells (New England Biolabs, Ipswich, USA; Catalog No. C3020), recovered at 37°C in 2 mL super optimal broth with catabolite repression (S.O.C.) .. 16-18 hours later, cells were harvested by centrifugation at 8,000 g. We then purified the PYL1 library plasmid DNA using the Plasmid Plus Midi Kit (New England Biolabs, Ipswich, USA; Catalog No. 12945).

    Transformation Assay:

    Article Title: The genetic architecture of an allosteric hormone receptor.
    Article Snippet: .. All four PYL1 mutant libraries were electrically transformed into high-efficiency, 10-beta electrocompetent E. coli cells (New England Biolabs, Ipswich, USA; Catalog No. C3020), recovered at 37°C in 2 mL super optimal broth with catabolite repression (S.O.C.) .. 16-18 hours later, cells were harvested by centrifugation at 8,000 g. We then purified the PYL1 library plasmid DNA using the Plasmid Plus Midi Kit (New England Biolabs, Ipswich, USA; Catalog No. 12945).

    Article Title: Refining chimeric antigen receptors via barcoded protein domain combination pooled screening
    Article Snippet: The product was digested with either MfeI or EcoRI, then the subpool that provides the backbone was processed using Quick CIP (New England Biolabs) and the backbone and insert were gel-purified. .. The insert was ligated into the backbone using T4 Ligase (New England Biolabs), then 1 mL was transformed into 10-beta electrocompetent E. coli cells (New England Biolabs). .. Cells were plated onto LB agar plates with ampicillin and zeocin selection, colonies scraped, and plasmids extracted with GenElute HP Plasmid Maxiprep Kit (Sigma-Aldrich).

    Article Title: Regulatory landscape of Alzheimer’s disease variants in human microglia
    Article Snippet: .. The fragments were inserted into the pLS-SceI vector (Addgene #137725) between SbfI and AgeI enzyme cutting site and then transformed into NEB 10-beta Electrocompetent E. coli cells (NEB C3020K) via electroporation. .. Bacterial stock containing a mixture of the library were grown overnight on Carbenicillin-positive plates (Teknova L5010) and maxiprepped for plasmid collection (Invitrogen K210017).

    Article Title: Mapping the GCK–GKRP interaction landscape using deep mutational scanning by reverse two-hybrid screening
    Article Snippet: .. Subsequently, 4 μL of each regional LR reaction was transformed into 100 μL NEB 10-beta electrocompetent E. coli cells using electroporation. ..

    Electroporation:

    Article Title: Regulatory landscape of Alzheimer’s disease variants in human microglia
    Article Snippet: .. The fragments were inserted into the pLS-SceI vector (Addgene #137725) between SbfI and AgeI enzyme cutting site and then transformed into NEB 10-beta Electrocompetent E. coli cells (NEB C3020K) via electroporation. .. Bacterial stock containing a mixture of the library were grown overnight on Carbenicillin-positive plates (Teknova L5010) and maxiprepped for plasmid collection (Invitrogen K210017).

    Article Title: Mapping the GCK–GKRP interaction landscape using deep mutational scanning by reverse two-hybrid screening
    Article Snippet: .. Subsequently, 4 μL of each regional LR reaction was transformed into 100 μL NEB 10-beta electrocompetent E. coli cells using electroporation. ..

    Purification:

    Article Title: Spike mutations that affect the function and antigenicity of recent KP.3.1.1-like SARS-CoV-2 variants
    Article Snippet: .. 1 μl of purified assembly was then used to electroporate NEB® 10-beta Electrocompetent E. coli cells (C3020K). ..

    Article Title: Spike mutations that affect the function and antigenicity of recent KP.3.1.1-like SARS-CoV-2 variants
    Article Snippet: .. 1 μl of purified assembly was then used to electroporate NEB ® 10-beta Electrocompetent E. coli cells (C3020K). ..

    Clone Assay:

    Article Title: Dissecting functional regulatory convergence over 160 million years of therian evolution
    Article Snippet: We used the NEB HiFi DNA Assembly Master Mix (E2621S, NEB) to assemble the final plasmid, with a 1:2 ratio of vector to insert. .. The reporter gene cloned plasmid was electroporated into 10-beta electrocompetent E. coli cells (C3020K, NEB) with the recommended settings (2.0 kV, 200 Ω and 25 μ F) and plated in LB agar plates containing 100 ug/mL of ampicillin. ..

    Plasmid Preparation:

    Article Title: Dissecting functional regulatory convergence over 160 million years of therian evolution
    Article Snippet: We used the NEB HiFi DNA Assembly Master Mix (E2621S, NEB) to assemble the final plasmid, with a 1:2 ratio of vector to insert. .. The reporter gene cloned plasmid was electroporated into 10-beta electrocompetent E. coli cells (C3020K, NEB) with the recommended settings (2.0 kV, 200 Ω and 25 μ F) and plated in LB agar plates containing 100 ug/mL of ampicillin. ..

    Article Title: Viral delivery of an RNA-guided genome editor for transgene-free germline editing in Arabidopsis.
    Article Snippet: .. For the bacterial interference assay, we co-transfected 100 ng of the TnpB-ωRNA plasmid and 100 ng of the target plasmid to 33 μl of NEB 10-beta electrocompetent E. coli cells (C3020K). ..



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